Abstract
An immunosensor based on screen-printed carbon electrode (SPCE) was explored to detect human parathyroid hormone (PTH). The SPCEs that were modified with nanocomposite containing gold nanoparticle (AuNP) and multi-walled carbon nanotube (MWCNT) were successfully prepared using electrochemical deposition. With theses SPCEs, we explored the fabrication of a novel, disposable and sensitive immunosensor comprising nanocomposite, antibody, and horseradish peroxidase. The nanocomposite such as AuNP and MWCNT not only provided a large surface area for antibody immobilization, but it also enhanced the electrochemical signal on the modified SPCE. The nanocomposite modified SPCE was characterized in detail.The label-free impedimetric and amperometric measurements were not affected by interference from the components presented in serum. This PTH immunosensor showed the detection limit of 0.033 and 0.092 pg/mL for impedimetric and amperometric methods. We provided a quick analysis of PTH in serum, which can potentially be used as the electrochemical immunosensing platform for point-of-care testing.
Published Version
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