Abstract
A electrochemical detection method for DNA hybridization has been developed and tested in an advanced analytical chemistry course. A set of experiments was designed to explore several concepts relevant to electropolymerization of intrinsically conducting polymers, their use as an electrically controlled ion-exchanger of chloride ions, and their ability to bind oligonucleotides through a noncovalent interaction with magnesium ions used as a linker between the polymer and the DNA strand. The electrostatic barrier on the modified electrode created by the phosphate groups of the probe DNA attached to the magnesium ion is modified by DNA hybridization. Changes in the transfer rate of chloride ions of the label-free DNA detector are detected as variations in cyclic voltammograms. The basic approach for the detection of the DNA hybridization event relies on the subtraction of cyclic voltammograms recorded before and after DNA hybridization. Different aspects of the experiments can be emphasized, dep...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
