La espectrometría de masas Maldi-Tof como técnica de rutina para la identificación de micobacterias típicas y atípicas en el laboratorio de microbiología clínica

Abstract

Introduction and aim: in the Mycobacterium genus have been described more than 120 species of Mycobacteria other than growth slow and fast. These microorganisms produce significant morbidity in humans, including type pulmonary infections, skin and soft tissues and disseminated disease being the rapid and precise is of great importance. Material and methods: the study population of our work were 75 isolated Mycobacteria from cultivation in solid Lowenstein-Jensen medium. The diagnosis was made by molecular techniques of PCR and reverse hybridization: GenoType Mycobacterium CM and GenoType Mycobacterium AS. Parallel strains were identified by mass spectrometry MALDITOF MS (Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry). Results: The overall agreement of results between the identification made by PCR and MALDI-TOF technology was 97, with a coefficient of correlation of 0.929 kappa (excellent correlation between 0.081-1.0). Conclusions: Our results indicate that it is quite feasible to incorporate MALDI-TOF MS for routine identification of Mycobacteria in the clinical microbiology laboratory workflow.