Abstract
Backgroundl-carnitine (LC) plays an important role in the oxidative/antioxidative balance of different organs. The study has explored the effect of the daily administration of aspartame (ASP) at two different doses for 4 weeks induces oxidative stress, inflammation, and histopathology in the brain of rats. This study also focused on explaining whether l-carnitine (LC) has potential ameliorative effects against pathophysiology induced by ASP in the brain. Adult male Wistar rats were split into six groups as follows: control animals were administered with vehicle, group of ASP at a low dose (ASP-LD) was given 75 mg/kg B.W., high dose of ASP (ASP-HD; 150 mg/kg), animals were treated with LC (10 mg/kg), ASP-LD + LC group and ASP-HD + LC treated rats.ResultsASP induced large increments in cytokines of interleukin-6, tumor necrosis factor-α, myeloperoxidase, xanthine oxidase, cyclooxygenase-2, and prostaglandin E2 in a dose-dependent response of the brain. Activities of superoxide dismutase/catalase, glutathione peroxidase, and acetylcholinesterase, besides levels of thiol, glutathione, a brain-derived neurotrophic factor, and neurotransmitters, were decreased in the brain tissue. The inauguration of brain injury by ASP administration was depended on the dose of treatment. Co-administration of LC with ASP significantly reversed and corrected all the abovementioned parameters.ConclusionThe data confirm that LC attenuated ASP neurotoxicity by suppressing the inflammation, oxidative stress and improving the neurotransmitters, antioxidants coupled with the histological and cellular structure.
Highlights
L-carnitine (LC) plays an important role in the oxidative/antioxidative balance of different organs
The study protocol was approved by the Animal Welfare Act and the Guide for the Care and the Use of Laboratory Animals established by Zagazig University, Zagazig, Egypt
Biochemical assessment MPO and Xanthine oxidase (XO) activities were the increment in ASPtreated groups and they decreased when the rats are treated with LC before ASP (Table 1)
Summary
L-carnitine (LC) plays an important role in the oxidative/antioxidative balance of different organs. Aspartame (ASP) consists of two amino acids, L-aspartic and L-phenylalanine It is an advocate by the Food and Drug Administration (FDA) in 1981 (Abdallah, 2002). It is commonly used among diverse foods, medicines, and hygiene products as artificial sweeteners (Abhilash, Paul, Varghese, & Nair, 2011; Mourad & Noor, 2011; NostiPalacios et al, 2014; Ashok, Sheeladevi, & Wankhar, 2015; Choudhary & Devi, 2015; Prakash, Jaspreet Kaur Kalra, & Kumar, 2015).
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