Kuraklık Stresi Altındaki Buğday Çeşitlerinde Salisilik Asit Uygulamalarının miRNA İfadesi Üzerine Etkileri

Abstract

Wheat cultivation in Turkey; is highly affected by environmental factors, such as drought stress, which reduces yields significantly. Applying chemicals that stimulate plant growth is an easy, low-cost, low-risk and effective approach to increase stress tolerance, and salicylic acid is prominent in adapting plants to adverse environmental conditions such as drought stress, salinity, and improving plants' tolerance to stress. In the study, Gün91 was used as drought tolerant variety and Ankara98 was used as the sensitive variety and two different doses (0.1 mM, 0.5mM) of salicylic acid were applied to the plants. Drought stress application was performed one week after salicylic acid application and RNAs were isolated from leaf samples collected from application and control pots, and expression levels of miR156, miR169, miR172, miR319 and mir398 and target genes belonging to these miRNAs were determined. While the expression level of miR156 and miR172 was higher in Day91 with tolerance to drought stress in leaf tissues treated with salicylic acid under drought stress conditions; the level of miR169 was higher in the sensitive Ankara98 variety. In Gün91 variety, miR319 expression level was higher in 0.5 mM salicylic acid + drought application, whereas in Ankara98 variety was higher in 0.1 mM salicylic acid + drought application. The miR398 expression level showed a significant decrease in both Gün91 and Ankara98 varieties in the plant groups where salicylic acid and drought stress were applied together compared to the control group. As a result; with this study, it was emphasized that salicylic acid is an important signal molecule in response to stress and it mitigates the effect in stress conditions, and the tolerance mechanism of drought tolerant and sensitive varieties with salicylic acid application were different.