Kualitas Sperma Beku Sapi Bali dalam Pengencer Air Kelapa Modifikasi dengan Berbagai Aras Dimethyl Sulfoxide (FROZEN SPERM QUALITY OF BALI BULLS IN MODIFIED COCONUT WATER EXTENDER WITH DIFFERENT DIMETHYL SULFOXIDE CONCENTRATION)

Heri Armadianto,Kirenius Uly,Thomas Mata Hine,Wilmientje Marlene Nalley

doi:10.19087/jveteriner.2019.20.1.93

Abstract

Dimethyl Sulfoxide (DMSO) is one type of cryoprotectant which has a low molecular weight so that it is easier to enter cells when cryopreservation. The purpose of this study was to explore the optimal concentration of DMSO in modified coconut water (mCW) extender that were able to maintain frozen sperm quality of bali bulls. Semen was collected from two four-year old bali bulls by artificial vagina. Good quality semen diluted with mCW (young coconut water + 20% egg yolk + 7.5 % moringa leaf extract) and supplemented by 3, 5, or 7% DMSO. Semen was filled into 0.25 ml ministraw, and was incubated in a refrigerator at 5°C for four hours, frozen on the surface of liquid nitrogen for 10 minutes and then dipped into liquid nitrogen. The quality of post thawing sperm was measured 24 hours later by placing the ministraw of frozen semen into water at 37oC for 30 seconds. Data were analyzed by analysis of variance and continued with Duncan test. Postthawing observations showed that bali bulls sperm cryopreserved at 3% DMSO yielded higher motility and viability (p<0.05) i.e. 36 and 44.15%, than DMSO 5% i.e. 18 and 23.65%, and DMSO 7% i.e. 7 and 12.62%. The recovery rate of sperm cryopreserved at 3% DMSO was also higher (p<0.05) than DMSO 5 and 7%, successively 45.65, 23.06, and 8.86%. The results of this study concluded that the optimal concentration of DMSO in mCW diluent to maintain frozen sperm quality of bali bulls was 3%.

Highlights

Dimethyl Sulfoxide (DMSO) is one type of cryoprotectant which has a low molecular weight so that it is easier to enter cells when cryopreservation
The purpose of this study was to explore the optimal concentration of DMSO in modified coconut water extender that were able to maintain frozen sperm quality of bali bulls
Semen was collected from two four-year old bali bulls by artificial vagina

Summary

METODE PENELITIAN

Materi yang digunakan dalam penelitian adalah semen yang ditampung dari dua ekor sapi bali jantan berumur empat tahun, milik Yayasan Williams dan Laura, di Kabupaten Kupang, Propinsi Nusa Tenggara Timur. Pembuatan Pengencer AKm Bahan-bahan yang digunakan untuk membuat pengencer semen adalah air kelapa, kuning telur dan ekstrak daun kelor (Moringa oleifera). Setelah disaring diperoleh ekstrak daun kelor yang siap untuk digunakan. Pembuatan pengencer AKm yaitu mencampur air kelapa dengan kuning telur dan ekstrak daun kelor dengan perbandingan 72,5:20:7,5 dan selanjutnya ditambahkan penisilin 1000 IU dan streptomisin 1 mg mL-1 pengencer. Setelah tercampur secara merata ditambahkan DMSO dengan konsentrasi 3%, 5%, atau 7%, dan disimpan di dalam lemari pendingin pada suhu 5oC. Semen yang layak digunakan untuk penelitian yaitu konsentrasi di atas 500x106 mL-1, motilitas di atas 70% dan abnormalitas sperma di bawah 20% (Hine et al, 2014). Kualitas sperma postthawing diukur 24 jam kemudian dengan menempatkan straw semen beku ke dalam air bersuhu 37oC selama 30 detik. Rancangan Percobaan dan Analisis Data Rancangan yang digunakan dalam penelitian ini adalah rancangan acak lengkap.

HASIL DAN PEMBAHASAN

Findings

DAFTAR PUSTAKA