Abstract
Boer goat have recently been popularly used for cross breeding with local goats. However, it is currently considered a breed at very limited number with relatively high prices . In this context, the cryopreservation of spermatozoa is important because it could be conserved for a very long period of time. Egg yolk extenders are most commonly used for cryopreservation of goat sperm. The aim of this study was to compare the ability of two extenders to maintain sperm viability after cryopreservation. Semen from three male Boer goat aged about 2-3 years old was collected using artificial vagina and frozen with Tris and Triladyl extender. The results showed that percentage of motility, viability and membrane integrity of spermatozoa with Tris and Triladyl extenders at every stage of cryopreservation showed not significantly difference (P>0.05), except the percentage of sperm motility post thawing of Triladyl was higher than Tris extender (52.00±4.47% vs 47.50±2.74%, P<0.05). Cryopreserved semen in Tris extender provided the same fertility rates after cervical insemination compared to Triladyl (62.50% vs 60.00%). In conclusion, the Tris extender has the same capabilities to Triladyl in cryopreservation of Boer goat spermatozoa as to maintain sperm quality and fertility rates. Key Words : Boer Goat, Spermatozoa, Cryopreservation, Fertility
Highlights
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The results showed that percentage of motility, viability and membrane integrity of spermatozoa with Tris and Triladyl extenders at every stage of cryopreservation showed not significantly difference (P>0.05), except the percentage of sperm motility post thawing of Triladyl was higher than Tris extender (52.00±4.47% vs 47.50±2.74%, P
Cryopreserved semen in Tris extender provided the same fertility rates after cervical insemination compared to Triladyl (62.50% vs 60.00%)
Summary
Penelitian ini dilakukan di Laboratorium Reproduksi Loka Penelitian Kambing Potong Sei Putih, Sumatera Utara. Materi ternak yang digunakan dalam penelitian ini adalah kambing pejantan Boer berjumlah 3 ekor yang berumur antara 2-3 tahun dengan bobot badan berkisar antara 45-50 Kg. Kambing ditempatkan dalam kandang individu yang dilengkapi dengan tempat pakan dan minum. Pemberian sumber bahan makanan dalam bentuk konsentrat dan hijauan pakan ternak. Pemberian konsentrat berkisar antara 300-500 gram per ekor per hari dilakukan pada waktu pagi hari, sedangkan hijauan pakan berupa rumput dengan jumlah pemberian berkisar antara 3-4 kg segar per ekor per hari diberikan pada waktu siang dan sore hari.
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