Abstract
Objective KRAS is the most frequently mutated oncogene in human neoplasms and we have previously reported KRAS p.G12V mutations in adenomatoid odontogenic tumors (AOT). We aimed to expand this cohort of samples and to test the association of KRAS mutations with clinical and histopathological parameters. A convenience sample of 30 AOT cases was included in the study. The hotpot KRAS p.G12V mutation was assessed by TaqMan allele-specific qPCR and codon 12 was direct sequenced. Clinical information obtained included patients age, tumor site, association of the lesion with impacted teeth and clinical tumor size. In addition, tumor capsule thickness was evaluated by morphometric analysis. Statistical analysis was carried out to test the association of KRAS codon 12 mutations with clinico-pathological parameters. Findings Molecular results confirmed KRAS p.G12V mutation in 14/23 cases, and p.G12R in 1/23. Eight cases were wild-type and samples from 7 cases failed amplification. Codon 12 mutations were not associated with any of the clinicopathological parameters tested (p>0.05). Conclusion AOT show high frequency of KRAS codon 12 mutations (15/23, 65%), which occur irrespectively of patients’ age, tumor location, association with impacted teeth, tumor clinical size or histopathological capsule thickness. Supported by FAPEMIG, CAPES and CNPq/Brazil.
Published Version
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