Abstract
BackgroundThe ethanol extract of KOTMIN13, composed of Inula japonica Flowers, Trichosanthes kirilowii Semen, Peucedanum praeruptorum Radix, and Allium macrostemon Bulbs, was investigated for its anti-asthmatic and anti-allergic activities.MethodsThe anti-asthmatic effects of KOTMIN13 were evaluated on ovalbumin (OVA)-induced murine asthma model. Anti-allergic properties of KOTMIN13 in bone-marrow derived mast cells (BMMC) and passive cutaneous anaphylaxis (PCA) in vivo were also examined.ResultsIn asthma model, KOTMIN13 effectively suppressed airway hyperresponsiveness induced by aerosolized methacholine when compared to the levels of OVA-induced mice. KOTMIN13 treatment reduced the total leukocytes, eosinophil percentage, and Th2 cytokines in the bronchoalveolar lavage fluids in OVA-induced mice. The increased levels of eotaxin and Th2 cytokines in the lung as well as serum IgE were decreased by KOTMIN13. The histological analysis shows that the increased inflammatory cell infiltration and mucus secretion were also reduced. In addition, the degranulation and leukotriene C4 production were inhibited in BMMC with IC50 values of 3.9 μg/ml and 1.7 μg/ml, respectively. Furthermore, KOTMIN13 treatment attenuated mast-mediated PCA reaction.ConclusionsThese results demonstrate that KOTMIN13 has anti-asthmatic and anti-allergic effects in vivo and in vitro models.
Highlights
The ethanol extract of KOTMIN13, composed of Inula japonica Flowers, Trichosanthes kirilowii Semen, Peucedanum praeruptorum Radix, and Allium macrostemon Bulbs, was investigated for its anti-asthmatic and anti-allergic activities
We investigated the effects of KOTMIN13 on the treatment of asthma in vivo model as well as allergic response by measuring inflammatory mediators in bonemarrow derived mast cells (BMMC) and passive cutaneous anaphylaxis (PCA) in mice
We showed that these effects were associated in part with the suppression of activated mast cells by inhibiting degranulation and eicosanoid production in bone-marrow derived mast cells (BMMC) as well as PCA in vivo
Summary
The ethanol extract of KOTMIN13, composed of Inula japonica Flowers, Trichosanthes kirilowii Semen, Peucedanum praeruptorum Radix, and Allium macrostemon Bulbs, was investigated for its anti-asthmatic and anti-allergic activities. The immediate reaction occurs within minutes caused by release of preformed mediators from basophils and mast cells upon allergen exposure. The late phase allergic reaction is caused by mobilization and accumulations of inflammatory cells, resulting further release of pharmacologically active mediators, sustaining the allergic response and promoting the late allergic response [1]. Mast cells are widely distributed throughout human respiratory tract and found in alveoli walls [2]. They are major effector cells that play a role in allergic inflammation. When activated through IgE-dependent or IgEindependent ways, mast cells release preformed mediators (histamine, proteases, and proteglycans) from their granules, lipid mediators (leukotrienes (LTs) and prostaglandins
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