KMP025 Application of a quantitative fragment analysis technique (T-RFLP) technique in medical microbiology

Abstract

Based on epidemiological and research evidence, HPV has a causal role in cervical carcinogenesis. Several HPV detection methods exist to date; the most commonly used method for detection of genital HPVs consists of nested PCR using the MY09/11 and GP5+/6+ primer sets (MY/GP+). Recently, the PGMY09/11 primer set, a modified version of the MY09/11 primer set, was introduced for single PCR and was found to detect a wider range of HPV types. The next logical step was taken and the efficacy of nested PCR using the PGMY09/11 and GP5+/6+ primer sets (PGMY/GP+) to detect HPV in cervical samples was evaluated.In this comparative study, nested PCR using the novel PGMY/GP+ primer set combination was found to be more type sensitive than the nested PCR with the MY/GP+ primer sets, detecting a wider range of HPV types, low copy HPVs, and better characterizing samples infected with multiple strains of HPV. Standardization and use of the PGMY/GP+ PCR system could aid physicians in providing more efficient HPV screening and better treatment for patients.