Abstract
Vector pNOM102-GOX was engineered to cloning gene gox in P. adametzii LF F-2044.1 and conditions were optimized for production of fungal protoplasts. Electroporation of P. adametzii LF F-2044.1 was conducted and transformants resistant to antibiotic geneticin were selected. Strains P. adametzii LF F-2044.1.17 and P. adametzii LF F-2044.1.18 displayed increased levels of glucose oxidase synthesis - mycelium productivity rose 2 - 2.5 times. It was found that efficient preservation of vectors in the transformants requires maintenance on the media containing antibiotic geneticin.
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