Abstract
This study evaluated the action of Pfaffia paniculata K., Juglans regia L., and Rosmarius officinalis L. extracts against planktonic form and biofilm of Klebsiella pneumoniae (ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at 107 UFC/mL of K. pneumoniae was distributed into 96-well microplates (n = 10) and after 48 h at 37°C and biofilm was subjected to treatment for 5 min with the extracts at a concentration of 200 mg/mL. ANOVA and Tukey tests (5%) were used to verify statistical significant reduction (p < 0.05) of planktonic form and biofilm. P paniculata K., R. officinalis L., and J. regia L. showed reductions in biomass of 55.6, 58.1, and 18.65% and cell viability reduction of 72.4, 65.1, and 31.5%, respectively. The reduction obtained with P. paniculata and R. officinalis extracts was similar to the reduction obtained with chlorhexidine digluconate 2%. In conclusion, all extracts have microbicidal action on the planktonic form but only P. paniculata K. and R. officinalis L. were effective against biofilm.
Highlights
Klebsiella pneumoniae belongs to Enterobacteriaceae family and it has emerged as an important pathogen, responsible for nosocomial infections, focusing on urinary (UTIs) and respiratory tracts and blood infections
Taking into consideration what has been exposed before, this study aimed to evaluate the antimicrobial action of glycolic extracts of P. paniculata K, J. regia L., and R. officinalis L. against K. pneumoniae planktonic suspension and biofilm
Glycolic extracts of Pfaffia paniculata, Juglans regia, and Rosmarinus officinalis were purchased from Mapric company with the appropriate reports and specifications
Summary
Klebsiella pneumoniae belongs to Enterobacteriaceae family and it has emerged as an important pathogen, responsible for nosocomial infections, focusing on urinary (UTIs) and respiratory tracts and blood infections. It is a common colonizer of skin, gastrointestinal tract, and nasopharynx, it is a risk for patients hospitalized for long periods and with compromised immune system. Virulence factor that makes it an extremely resistant microorganism, it offers great resistance to the diffusion of antimicrobial drugs [1,2,3,4], with little or no treatment option being developed [5]. The extracts selected for this study show little or no information regarding their antimicrobial potential [6,7,8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
