Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates)

Lucas De Paula Ramos,Leandro Wagner Figueira,Maria Angélica De Sá Assis,Felipe Eduardo Oliveira,Carlos Eduardo Rocha Santos,Amanda Romangnoli,Marcela Dos Santos Oliveira,Thaís Cristine Pereira,Luciane Dias De Oliveira,João Manoel Theotônio Dos Santos,Patrícia Michelle Nagai De Lima,Suelen Simões Amaral

doi:10.33448/rsd-v10i12.20270

Lucas De Paula Ramos, Leandro Wagner Figueira + Show 10 more

Open Access

https://doi.org/10.33448/rsd-v10i12.20270

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Abstract

Objective: Evaluate the anti-biofilm activity of Gymnema sylvestre, Hamamelis virginiana, Juglans regia, Persea americana, Pfaffia paniculata, Rosmarinus officinalis, Stryphnodendron barbatiman and Thymus vulgaris extracts in 3 MDR strains and 1 ATCC (4352) of Klebsiella pneumoniae. Methods: First were made a screening with broth microdilution test, protocol M7-A9, according to CLSI. Extracts that presented values for Minimal Microbicidal Concentration (MMC) were selected for biofilms tests on 96 wells plates. Treatments used concentrations of 25 and 50 mg/mL, after biofilms were measured by biomass and MTT tests and statistically analyzed by ANOVA and Tukey test (p<0.05). Results: All extracts showed MIC for all K. pneumoniae strains studied, with values ranging from 12.5-100 mg/mL. Biomass of the strains ATCC and MDR strain (400381) of K. pneumoniae obtained reductions of 37.7 and 44.3% with P. paniculata and R. officinalis extracts. The isolate 386546 obtained a reduction of 29.7% (p<0.05) under R. officinalis action. Conclusion: Among the extracts studied, those that were most effective in the anti-biofilm action were J. regia, P. paniculata and R. officinalis. Therefore, these extracts could act as bactericidal agents against K. pneumoniae MDR.

Highlights

Klebsiella pneumoniae is a Gram-negative bacillus in the family of enterobacteria
Among the extracts studied, those that were most effective in the anti-biofilm action were J. regia, P. paniculata and R. officinalis
Considering the world scenario of microbial resistance and the wide variety of plant extracts with potential to be explored, the present study evaluated the antimicrobial action of Gymnema sylvestre (Gimena), Hamamelis vir-giniana L. (Hamamelis), Juglans regia L. (Walnut ), Persea americana (Avocado), Pfaffia paniculata (Brazilian Ginseng), Rosmarinus officinalis L. (Rosemary), Stryphnodendron barbatiman (Barbatiman) and Thymus vulgaris (Thyme) in planktonic forms and biofilms of Klebsiella pneumoniae MDR strains

Summary

Introduction

Klebsiella pneumoniae is a Gram-negative bacillus in the family of enterobacteria It is an opportunistic pathogen, present in 70% of hospital infections, and causes mainly pulmonary and urinary tract infections (Bowers et al, 2016; Rahim et al, 2016). K. pneumoniae may be prevalent as an infectious agent in patients with urinary catheters and may develop bacterial biofilms in these devices (Djeribi, Bouchloukh, Jouenne, & Menaa, 2012; Ramstedt et al, 2019) This virulence mechanism is fundamental in many bacterial infections, as it can resist the host's defense system and antimicrobial treatments (Djeribi et al., 2012; Vuotto, Longo, Balice, Donelli, & Varaldo, 2014). In addition to the ability to form biofilms, the species has other virulence factors, such as polysaccharide capsules, siderophore, urease, the presence of fimbriae and resistance to antibiotics, in which they help in the maintenance and resistance of infections (Bowers et al, 2016; Clegg & Murphy, 2016; Rahim et al, 2016).

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