Kinetics of photoautotrophic growth of Marchantia polymorpha cells in suspension culture

Abstract

Chlorophyllous, cultured cells of Marchantia polymorpha L. (HYA‐2 cell line) grow actively under photoautotrophic (lithotrophic) conditions. The maximum specific growth rate (μcell) was 0.64 day−1 and the doubling time was 1.08 days under optimum conditions (165 μmol m−2 s−1, 1% carbon dioxide enriched atmosphere, 25°C). The photosynthetic activity was 1.30 μmol CO2‐fixed (106 cells)−1 h−1 [66 μmol (mg chlorophyll)−1 h−1] in the exponential phase. The growth course has two distinct phases, an exponential and a linear one. The exponential phase is observed as long as the population density is sufficiently low (less than 7.9 × 106 cells ml−1), so that practically all individual cells directly receive the full incident light. The effect of light on the specific growth rate is a linear function of photon flux density. Linear growth occurs after the population density is so high that the incident light is almost completely absorbed by the cell suspension. The growth rate is a logarithmic function of photon flux density, in contrast to the specific growth rate, and saturates at high photon flux densities. The conditions of maximum growth, however, are not wellbalanced between cell mass production and cell division. Therefore, the maximum growth does not continue for a long time.