Kinetics of nutrients remediation from sugar industry effluent-treated substrate using Agaricus bisporus: mushroom yield and biochemical potentials.

Abstract

This study investigated the yield and biochemical potential of Agaricus bisporus mushroom cultivated on agricultural waste substrate supplemented with treated sugar industry effluent (SIE). Laboratory-scale experiments were performed for the cultivation of A. bisporus on a mixture of wheat straw and sugar cane bagasse moistened with different doses of borewell water (BWW) and treated SIE (0-100%). Besides this, the simultaneous effects of the SIE amendment on total Kjeldahl's nitrogen (TKN) and total phosphorus (TP) contents of substrate and kinetics of their utilization by A. bisporus were studied. Results showed a relatively higher utilization of TKN (38.10 ± 1.60%) and TP (47.4 ± 6.44%) in a 25:75 ratio of BWW and SIE, respectively. The kinetics studies of TKN and TP utilization using Lineweaver-Burk models described the maximum specific utilization rates (V max) of 0.165 and 0.125mg·kg-1·d-1 and saturation points (K m ) of 72.401 and 33.283mg·kg-1, respectively, which are in good agreement as indicated by R 2 values (> 0.90). In addition, the maximum significant (P < 0.01) yield (159.31 ± 8.85g·Kg-1), biological efficiency (106.21 ± 3.84%), total phenols (3.03 ± 0.07mg·g-1), ascorbic acid (0.44 ± 0.03mg·g-1), and β-carotene (3.36 ± 0.05μg·g-1) of A. bisporus were observed using the same treatment. Therefore, this paper reported sustainable utilization of TKN and TP nutrients from SIE for A. bisporus mushroom cultivation.