Abstract

The time course of labelling of the various classes of viruslike particles that can be isolated from plants infected with turnip yellow mosaic virus has been followed using S 35 to label protein and P 32 to label RNA. A theoretical treatment for the relation between virus protein and nucleoprotein is presented. The labelling data eliminate certain of the theoretically possible relationships, but a number of alternatives remain. The simplest model consistent with the data is one in which the protein is formed reversibly and the nucleoprotein irreversibly from the same pool of precursor protein subunits. The minor nucleoprotein components cannot be stages in the assembly between the empty protein shell and complete virus. Various attempts to carry out pulse type labelling experiments with P 32 in leaves of Brassica pekinensis were unsuccessful.

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