Abstract
Abstract The kinetics of the oxidation of allyl alcohol by yeast alcohol dehydrogenase (YADH) was studied with stopped-flow technique. The experimental results showed that the specific activity of YADH remained constant within theYADH concentration range ofO-16.4mg/dm3 and YADH exhibited maximum activity at pH 7.9 and a phosphate buffer concentration of 0.25 mol/dm3 The oxidation rate of allyl alcohol by YADH followed the Arrhenius equation within the temperature range of 10 to 40°C The YADH was denatured irreversibly at the temperatures above 40°C. The rate equation for the YADH-catalyzed oxidation of allyl alcohol was obtained. A mixed-type substrate inhibition occurred when the concentration of allyl alcohol was higher than 200 mmol/dm3 The presence of LiCl, NaCl and KG could enhance the activity of YADH. The YADH lost activity significantly in the presence of organic solvents, particularly benzene and its derivatives.
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