Abstract
Soluble polymers bearing ribonuclease have been produced by a co- crosslinking method. The kinetic properties have been studied with the polymer in solution and gelified as a membrane, and the effects of chemical modification and of insolubilization studied separately. The results obtained have been compared with those generated by native enzyme and ribonuclease immobilized within an artificial membrane. Experiments have been performed with a high molecular weight substrate (RNA) and a low molecular weight substrate (cytidine-2′, 3′-phosphate). With the data obtained using these substrates, it is possible to evaluate the effects of the diffusion and accessibility constraints on the kinetic behavior of the enzyme.
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