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Abstract
Abstract A kinetic potentiometric method is described for the determination of peroxidases in vegetables and medicinal plants. It is based on the measurement of fluoride ion liberation rate from the peroxidase-catalyzed reaction of p-fluorophenol with hydrogen peroxide, sing the fluoride ion-selective electrode. The calibration curve is linear in the range 1-10 mU/mL with a precision of 1-3%. No interference was found from plant extracts, and recoveries were 97-107% (mean 100.9%). The method is rapid (2 min per measurement) and applicable to highly colored or turbid solutions. The method was applied for the determination of peroxidase activity in various plants of Greek flora. Plants of Leguminosae were found to be the richest source.
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