Abstract
Controlling initial bacterial adhesion is essential to prevent biofilm formation and implant-related infection. The search for surface coatings that prevent initial adhesion is a powerful strategy to obtain implants that are more resistant to infection. Tracking the progression of adhesion on surfaces from the beginning of the interaction between bacteria and the surface provides a deeper understanding of the initial adhesion behavior. To this purpose, we have studied the progression over time of bacterial adhesion from a laminar flow of a bacterial suspension, using a modified Robbins device (MRD). Comparing with other laminar flow devices, such as the parallel plate flow chamber, MRD allows the use of diverse substrata under the same controlled flow conditions simultaneously. Two different surfaces of Ti6Al4V and two strains of Staphylococcus epidermidis with different exopolymer production were tested. In addition, the modified Robbins device was examined for its convenience and suitability for the purpose of this study. Results were analyzed according to a pseudofirst order kinetic. The values of the parameters obtained from this model make it possible to discriminate the adhesive behavior of surfaces and bacteria. One of the fitting parameters depends on the bacterial strain and the other only on the surface properties of the substrate.
Highlights
Orthopedic replacements help increase life expectancy and improve health conditions, especially in elderly patients. e progress in implant design is constant
Several authors have analyzed the initial affinity between bacteria and substrates and, in many cases, this information provided a good clue for predicting the ability of a given material to resist bacterial colonization [18,19,20,21,22,23]
We found that the coating developed protects Ti6Al4V against bacterial adhesion under static conditions
Summary
Orthopedic replacements help increase life expectancy and improve health conditions, especially in elderly patients. e progress in implant design is constant. Several authors have analyzed the initial affinity between bacteria and substrates and, in many cases, this information provided a good clue for predicting the ability of a given material to resist bacterial colonization [18,19,20,21,22,23]. More relevant information on the initial interaction between the surface and the bacteria can be obtained if the adhesion takes place under flow. For this purpose we will follow the adhesion process on this material for two strains of S. epidermidis which that are expected to produce different biofilms due to their different EPS production capacity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
