Measurement of charge transfer during bacterial adhesion to an indium tin oxide surface in a parallel plate flow chamber

Abstract

An experimental method is described for the measurement of charge transfer during bacterial adhesion in situ to a transparent, semiconducting indium tin oxide (ITO) coated glass plate in a parallel plate flow chamber. Bacterial adhesion is measured simultaneously with either the electric potential or the capacitance of the surface. Initial bacterial adhesion was accompanied by a change in electric potential of the surface with no measurable change in capacitance. Consequently, it can be assumed that the change in electric potential of the surface is due to charge transfer between bacteria and the surface, and it can be calculated that, on average, a charge of about 10 −14 C per bacterium is exchanged during initial adhesion, which corresponds to only several percent of the total surface charge of a bacterium. Charge transfer could either be to or from the bacterial cell surface, dependent on the bacterial strain involved and the ionic strength used.