Abstract
One key advantage of label-free biosensors involves the monitoring of binding between biomolecules. However, a number of experimental artifacts may lead to complicated real-time curves that do not fit well to a simple Langmuir model. As a result, the quality of the kinetic data must be improved to obtain accurate reaction rates. By carefully designing experiments, collecting the data, and processing the data, issues arising from signal drift, nonspecific binding, mass-transport effect in solution, mass-transport effect on surface, and curve fitting may be avoided or resolved. Using a label-free oblique-incidence reflectivity difference biosensor, key processes required to obtain reliable kinetic data and accurate reaction rates are described.
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