Abstract
BackgroundKinesin family member 2a (KIF2A), a type of motor protein found in eukaryotic cells, is associated with development and progression of various human cancers. The role of KIF2A during breast cancer tumorigenesis and progression was studied.MethodsImmunohistochemical staining, real time RT-PCR and western blot were used to examine the expression of KIF2A in cancer tissues and adjacent normal tissues from breast cancer patients. Patients’ survival in relation to KIF2A expression was estimated using the Kaplan–Meier survival and multivariate analysis. Breast cancer cell line, MDA-MB-231 was used to study the proliferation, migration and invasion of cells following KIF2A-siRNA transfection.ResultsThe expression of KIF2A in cancer tissues was higher than that in normal adjacent tissues from the same patient (P < 0.05). KIF2A expression in cancer tissue with lymph node metastasis and HER2 positive cancer were higher than that in cancer tissue without (P < 0.05). A negative correlation was found between KIF2A expression levels in breast cancer and the survival time of breast cancer patients (P < 0.05). In addition, multivariate analysis indicated that KIF2A was an independent prognostic for outcome in breast cancer (OR: 16.55, 95% CI: 2.216-123.631, P = 0.006). The proliferation, migration and invasion of cancer cells in vitro were suppressed by KIF2A gene silencing (P < 0.05).ConclusionsKIF2A may play an important role in breast cancer progression and is potentially a novel predictive and prognostic marker for breast cancer.
Highlights
Kinesin family member 2a (KIF2A), a type of motor protein found in eukaryotic cells, is associated with development and progression of various human cancers
KIF2A mRNA was up-regulated in primary breast cancer tissue, by 2.95 fold the expression in adjacent normal breast tissues (Figure 1A, B, P < 0.001)
The western blotting analysis showed that the KIF2A protein levels in primary breast cancer tissues were markedly higher than those in the adjacent normal breast tissues (Figure 1C, D, P < 0.001)
Summary
Kinesin family member 2a (KIF2A), a type of motor protein found in eukaryotic cells, is associated with development and progression of various human cancers. It has been shown that the kinesin-13 class of proteins, which includes KIF2A, KIF2B, and KIF2C/MCAK, play an important role in mitosis [4,5]. These proteins modulate intracellular transport, cell division, and bipolar spindle assembly during spindle formation [5,6,7]. Monopolar spindles in the cells could cause the gain or loss of chromosomes in daughter cells [16] Any interference with this process can lead to chromosome missegregation, resulting in significant changes in the proliferation and migration of tumor cells. The goal of this study was to explore the function of KIF2A in human breast cancer, and to determine its effects on the proliferation and invasion of breast cancer cells
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