Kidins220/ARMS interacts with Pdzrn3, a protein containing multiple binding domains

Massimiliano Andreazzoli,Beatrice D’Orsi,Stephen W Wilson,Marianna Vitiello,Luciana Dente,Angela Iervolino,Manuela Barilari,Gaia Gestri,Elena Landi

doi:10.1016/j.biochi.2012.05.002

Massimiliano Andreazzoli, Beatrice D’Orsi + Show 7 more

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https://doi.org/10.1016/j.biochi.2012.05.002

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Journal: Biochimie	Publication Date: May 15, 2012
Citations: 8	License type: cc-by

Affiliation: University of Pisa, University College London

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We report the identification of a novel partner of Kidins220/ARMS (Kinase D-interacting substrate of 220kDa/Ankyrin Repeat-rich Membrane Spanning) an adaptor of neurotrophin receptors playing crucial roles during neurogenesis. Screening a phage display library of brain cDNA products we found that D.rerio Pdzrn3, a protein containing RING-finger and PDZ-domains, interacts with Kidins220/ARMS through its first PDZ-domain. Both zebrafish proteins share high homology with the corresponding mammalian proteins and both genes are developmentally expressed in neural districts where early neurogenesis occurs. The interaction was also confirmed by biochemical assays and by co-localization at the tips of growing neurites of PC12 cells induced with nerve growth factor.

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We report the identification of a novel partner of Kidins220/ARMS (Kinase D-interacting substrate of 220 kDa/Ankyrin Repeat-rich Membrane Spanning) an adaptor of neurotrophin receptors playing crucial roles during neurogenesis
Screening a phage display library of brain cDNA products we found that D. rerio Pdzrn3, a protein containing RING-finger and PDZ-domains, interacts with Kidins220/ARMS through its first PDZ-domain
Both zebrafish proteins share high homology with the corresponding mammalian proteins and both genes are developmentally expressed in neural districts where early neurogenesis occurs

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We report the identification of a novel partner of Kidins220/ARMS (Kinase D-interacting substrate of 220 kDa/Ankyrin Repeat-rich Membrane Spanning) an adaptor of neurotrophin receptors playing crucial roles during neurogenesis. Screening a phage display library of brain cDNA products we found that D. rerio Pdzrn3, a protein containing RING-finger and PDZ-domains, interacts with Kidins220/ARMS through its first PDZ-domain. Pdzrn3 expression has been characterized in mammalian muscle tissues, in murine C2C12 cells differentiated in myotubes or osteoblasts and more recently in the central nervous system of zebrafish embryos, where it could play a role in neurogenesis [2e5].

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