Abstract
Lucuma (Pouteria campechiana) is a fruit plant that is rich in biologically active substances such as carotenoids. In this study, the data of 20 lucuma samples in Can Tho city were collected and evaluated based on morphological characteristics and ISSR molecular markers. 50 morphological characteristics of lucuma were analyzed. 20 lucuma samples were divided into seven groups with the similarity coefficient from 0.71 to 0.86. The Shannon index showed that 20 lucuma samples had high diversity value which the highest value peaked at 1.526. Electrophoretic analysis by 8 ISSR markers also showed the significance of polymorphism. There were 115 out of 119 bands were polymorphic with the ratio of 96.36%. Each marker amplified an average of 14.88±4.29 bands, of which 14.38±4.5 bands were polymorphic. The similarity coefficient was from 0.41 to 1.00 and able to cluster into 4 separate groups. Finally, the combined results of morphological characteristics and molecular markers analysis illustrated that total 20 samples were classified into 4 groups with high similarity ranging from 0.63 to 0.90. The overall result of this study showed that 20 lucuma samples have considerable diversity. These are valuable original information resource for lucuma selection and breeding as well as support for further studies about the biochemical characteristics of lucuma.
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