Abstract
The existence of genetic diversity in turmeric (Curcuma sp.) is documented in other countries but not in Vietnam, where turmeric is an introduced species. This study is aimed to determine the possible existence of genotypic diversity among 20 turmeric accessions in Southern Vietnam using RAPD and ISSR markers. Analysis of ten RAPD markers (OPA02, OPA03, OPA04, OPA10, OPA13, OPB07, OPB10, OPD02, OPD03 and OPD07) showed a relatively high level of polymorphism: 133 out of total 154 bands were polymorphic, or a ratio of 89.7%. RAPD markers analysis showed the Euclidean distances ranging from 0-8.94 (with a mean of 6.87) and able to cluster into 5 groups. Analysis of ten ISSR markers (ISSR1, ISSR2, ISSR5, ISSR6, ISSR7, ISSR10, ISSR12, ISSR14, ISSR17 and ISSR18) also showed a relatively high level of polymorphism: 132 out of total 136 bands were polymorphic, or a ratio of 97.1%. ISSR markers analysis showed the Euclidean distances ranging from 1.73-8.54 (with a mean of 6.75) and able to cluster into 5 groups. A total of 292 bands was produced by the combined RAPD and ISSR markers and 272 bands (93.2%) were polymorphic. Using combined RAPD and ISSR markers showed the Euclidean distances ranging from 2.65-12.2 (with a mean of 9.65) and able to cluster into 4 groups. The overall results showed that these 20 turmeric accessions in Southern Vietnam had high levels of diversity.
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