Abstract
Diocalandra frumenti is the severely dangerous insect causing various problems to coconut in several provinces in the Mekong Delta of Vietnam. The genetic diversity was analyzed by applying ISSR marker (Inter-simple sequence repeats) as a molecular marker in order to make a specific picture of the population diversity of Diocalandra frumenti for further studies. In this research, 40 samples of adult insect were collected from 8 provinces in the Mekong Delta and 4 provinces in the southeast region. Four phenotypes were classified based on morphological characters, and ten ISSR primers were applied in the experiment to identify the genetic diversity. The results included 161 amplified fragments generated by 10 set of selected ISSR primers, of which 148 fragments were polymorphic (91.7%). Genetic relationship of 40 pests were clustered by UPGMA to demonstrate the differentiation of all species, showing an extensive genetic diversity ranged from 3.16 to 8.54. According to the diagram, 40 samples were grouped into four main clusters. This report illustrates the influence of geographical origin on genetic diversity.
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