Abstract
This study was carried out to determine the effects of extracted herbs as anti-fungal agents on Achlya sp. and Saprolegnia sp. The antifungal activity, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of five herbs including Chromolaena odorata, Achyranthes aspera, Carica papaya, Perilla frutescens and Muntingia calabura were examined for snakehead pathogenic Achlya sp. and Saprolegnia sp. The result indicated that extract of Perilla frutescens showed antifungal activity higher than those of the remaining herbs. MIC and MFC of Perilla frutescens showed the most effective against to both fungal hyphae and zoospore of Achlya sp. and Saprolegnia sp. in 1.6 mg/mL at 24 h exposure. Chromolaena odorata and Achyranthes aspera exhibited antifungal activity to hyphae and zoospores at concentrations of 3.2 mg/mL. The hyphae of Achlya sp. and Saprolegnia sp. were grown when they were exposed to 100, 50, 25, 12.5 and 6.4 mg/mL of Carica papaya solution.
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