Abstract
To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC. Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes. There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.
Highlights
Over 6 million people worldwide suffer a burn injury per year[1]
There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor
Four women with a mean age of 42.5 years were selected as controls and 4 (3 women and 1 man) burn patients and a mean age of 36 years were included in the study
Summary
Over 6 million people worldwide suffer a burn injury per year[1]. It is estimated that 1 million burn injuries occur annually only in the United States, accounting for 5,000 deaths in the same period. Burns are the fourth leading cause of death from unintentional injuries[2]. In Brazil, no official statistics provide estimates of burn incidence or mortality. Burn injuries are not rare and can be costly to the public health system[3]. Severe burn injury is a complex trauma associated with high morbidity and mortality, and immediate and long-term consequences, requiring intensive care in the acute treatment for restoration of hemodynamics, pain relief, preventing and treating sepsis, and skin replacement[3,4]. Burn patients need special care due to psychosocial sequelae[4]
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