Karyotype Analysis in seven cultivars ofNarcissusspp.

Abstract

We investigated the chromosome number and karyotype in seven cultivars of Narcissus spp. using the traditional squash method. The result indicated that all seven cultivars were diploid and the basic chromosome numbers in these seven cultivars were n = 12 and 14. The karyotype was 2B for all seven cultivars. Six of seven cultivars consisted of m and sm chromosome, and only one cultivar contains st chromosome. The detailed karyotypes of the seven cultivars are as follows: Arkle: 2n = 2x = 28 = 3m + 11sm, index of the karyotypic asymmetry (As·k) = 66.57 %; Fortissimo: 2n = 2x = 28 = 4m + 10sm, As·k = 66.60 %; Pink Charm: 2n = 2x = 28 = 3m + 10sm + 1st, As·k = 66.62 %; Mary Bcharmon: 2n = 2x = 24 = 5m + 7sm, As·k = 64.56 %; Barret Browing: 2n = 2x = 24 = 6m + 6sm, As·k = 65.73 %; Romance: 2n = 2x = 24 = 2m + 10sm, As·k = 65.64 %; Dutch Master: 2n = 2x =24 = 3m + 9sm, As·k = 65.95 %. Our results indicated that the karyotype analysis combined with morphological characters could assist to classify the taxonomic ...