Abstract
Cytogenetic studies on five Red Sea reef fish species Larabicus quadrilineatus, Hemigymnus melapterus,Thalassoma rueppellii, Thlassoma lunare and Gomphosus caeruleus of the family Labridae (Perciformes)were carried out; all samples were collected from Hurghada, on the Egyptian Red Sea coast. All thespecimens showed a diploid chromosome number of 2n=48, they were also identical in the karyotype (allacrocentric), and in the Fundamental number FN=48. The RAPD-PCR analysis were carried out by usingeight primers OPA12, 5`-TCGGCGATAG-3, OPA14,5`-TCTGTGCTGG-3`, OPA15, 5`-TTCCGAACCC-3`, OPA17,5`- GACCGCTTGT – 3`, OPA18 5`-AGGTGACCGT-3`, OPA19 5`-CAAACGTCGG-3`,OPA20,5`-GTTGCGATCC-3` and OPO11,5`- GACAGGAGGT- 3`.The results were extremely useful to improve the importance of using RAPD-PCR in genetic analysisamong the species which are identical in chromosome number and karyotype.
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