Abstract
Foshou San is a typical gynaecological formula with wild usage in traditional Chinese medicine. Jiawei Foshou San (JFS) is a novel ingredient prescription from Foshou San with antiendometriosis effect in unclear mechanisms. To uncover the potential application and proapoptotic mechanisms of JFS, JFS ingredient-drug target-disease networks, GO enrichment, and pathway analysis were established for potential application prediction. Molecular docking and validation in vivo were investigated by the proapoptotic mechanisms of JFS. In this study, 99 common targets were related to 108 diseases. 484 biological processes, 44 cell components, 59 molecular functions, and 37 pathways were significantly identified in GO enrichment and pathway analysis. In molecular docking, ligustrazine showed binding activity with Bcl-2, Bax, caspase-9, caspase-3, and PARP. In vivo, JFS elevated the shrink rate of ectopic endometrium, by suppressing E2 and PROG. An in-depth study showed that apoptosis was activated through diminishing Bcl-2, rising Bax and Bad, and expressing more caspase-3 and caspase-9 using JFS. JFS promoted the protein level of cleaved-PARP. In brief, JFS might be applied for various diseases through multiple targets and pathways, especially endometriosis by Bcl-2 pathway. These findings reveal the potential application for further evaluation and uncover the proapoptotic mechanism of JFS.
Highlights
Blood stasis syndrome in traditional Chinese medicine (TCM) is considered appearing in various chronic diseases, such as cardiocerebrovascular diseases, gynaecological diseases, tumor, and infectious diseases [1,2,3]
Common targets were screened by comparing Jiawei Foshou San (JFS) targets with FDA-approved drug targets. e connection between common targets and diseases was obtained from TTD and TCMSP. e diseases were classified according to MeSH
JFS Target-Drug Target Network Construction and Analysis. rough TCMSP, SEA, and BATMAN-TCM databases, there were 293 potential targets corresponding to JFS ingredients, including 10 for ligustrazine, 86 for ferulic acid, and 197 for tetrahydropalmatine (Table S1). 1215 FDAapproved drug targets were obtained from DrugBank and TTD databases (Table S2)
Summary
Blood stasis syndrome in traditional Chinese medicine (TCM) is considered appearing in various chronic diseases, such as cardiocerebrovascular diseases, gynaecological diseases, tumor, and infectious diseases [1,2,3]. Huoxue Huayu recipe is regarded to ameliorate the blood stasis syndrome through activating blood and dissolving stasis in TCM [1, 4]. Foshou San formula is a classic Huoxue Huayu recipe, utilized extensively in gynaecological diseases, cardiocerebrovascular diseases, and hepatobiliary disease [5]. Our previous research has found that JFS has a good therapeutic effect on endometriosis (EMS), including reducing the inflammatory response and antimetastasis [3, 6]. The proapoptotic mechanism of JFS has not been measured in EMS. It is worth to detect the potential application of JFS on disease belonging to blood stasis syndrome
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