ITRAQ protein profile analysis of sugar beet under salt stress: different coping mechanisms in leaves and roots

Junliang Li,Congyu Wang,Cuihong Dai,Dayou Cheng,Jie Cui,Tianjiao Liu,Chengfei Luo

doi:10.1186/s12870-020-02552-8

Abstract

BackgroundSalinity is one of the most serious threats to world agriculture. An important sugar-yielding crop sugar beet, which shows some tolerance to salt via a mechanism that is poorly understood. Proteomics data can provide important clues that can contribute to finally understand this mechanism.ResultsDifferentially abundant proteins (DAPs) in sugar beet under salt stress treatment were identified in leaves (70 DAPs) and roots (76 DAPs). Functions of these DAPs were predicted, and included metabolism and cellular, environmental information and genetic information processing. We hypothesize that these processes work in concert to maintain cellular homeostasis. Some DAPs are closely related to salt resistance, such as choline monooxygenase, betaine aldehyde dehydrogenase, glutathione S-transferase (GST) and F-type H+-transporting ATPase. The expression pattern of ten DAPs encoding genes was consistent with the iTRAQ data.ConclusionsDuring sugar beet adaptation to salt stress, leaves and roots cope using distinct mechanisms of molecular metabolism regulation. This study provides significant insights into the molecular mechanism underlying the response of higher plants to salt stress, and identified some candidate proteins involved in salt stress countermeasures.

Highlights

Salinity is one of the most serious threats to world agriculture
Activity of the roots was measured by monitoring Triphenyltetrazolium chloride (TTC); reducing capacity was 1.5-fold greater under salt stress compared to control plants (Fig. 1f)
Coverage with less than 10%, 10–30%, and 30–100% accounted for 57.6, 33.9 and 8.4% in leaves (Fig. S4a) and 55.6, 30.6 and 13.8% in Bioinformatics analysis of differential abundance protein species (DAPS) identified by Isobaric tags for relative and absolute quantitation (iTRAQ) The putative functions of salt stress-responsive Differentially abundant proteins (DAPs) were investigated using Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and Clusters of Orthologous Groups of proteins (COG) analyses (Fig. 3)

Summary

Introduction

An important sugar-yielding crop sugar beet, which shows some tolerance to salt via a mechanism that is poorly understood. Unlike other plant abiotic stresses, salinity causes both osmotic stress and ion toxicity via Na+ and Claccumulation [4] The latter may cause membrane disorganization, generation of reactive oxygen species (ROS), metabolic toxicity, inhibition of photosynthesis and attenuation of nutrient acquisition [5, 6]. Some salt tolerant varieties such as sugar beet Isobaric tags for relative and absolute quantitation (iTRAQ) [8,9,10] is one of the most reliable labeling techniques for proteome quantification, and has been used previously to analyze salt stress-induced proteome changes in sugar beet.

Methods

Results

Conclusion