Abstract
Microorganism degradation is efficient to remove the steroid hormones like 17β-estradiol (E2); but their degradation mechanism and metabolic network to these chemicals are still not very clear. Here the global responses of the estrogen-degradation strain Pseudomonas putida SJTE-1 to 17β-estradiol and glucose were analyzed and compared using the iTRAQ (isobaric tags for relative and absolute quantization) strategy combined with LC-MS/MS (liquid chromatography-tandem mass spectrometry). 78 proteins were identified with significant changes in expression; 45 proteins and 33 proteins were up-regulated and down-regulated, respectively. These proteins were mainly involved in the processes of stress response, energy metabolism, transportation, chemotaxis and cell motility, and carbon metabolism, considered probably responding to 17β-estradiol and playing a role in its metabolism. The up-regulated proteins in electron transfer, energy generation and transport systems were thought crucial for efficient uptake, translocation and transformation of 17β-estradiol. The over-expression of carbon metabolism proteins indicated cells may activate related pathway members to utilize 17β-estradiol. Meanwhile, proteins functioning in glucose capture and metabolism were mostly down-regulated. These findings provide important clues to reveal the 17β-estradiol degradation mechanism in P. putida and promote its bioremediation applications.
Highlights
It was found that P. putida KT2440 would induce a global response to aromatic hydrocarbon sources by up- or down-regulating series of enzymes for substrate uptake, transport, degradation, and product export[28,29]
We applied an iTRAQ-based quantitative proteomics technology to characterize the proteomic profiles of P. putida SJTE-1 in 17β-estradiol environment, compared to those in glucose condition. 78 proteins were found to be significantly dys-regulated, involved in the processes of stress responses, uptake and transport, energy metabolism, translation and nucleotide metabolism, and carbohydrate metabolism
To profile the expression of proteins induced by 17β-estradiol in P. putida SJTE-1, quantitative proteomic analysis based on an iTRAQ labeling method was executed
Summary
To profile the expression of proteins induced by 17β-estradiol in P. putida SJTE-1, quantitative proteomic analysis based on an iTRAQ labeling method was executed. Proteins involved in process of stress response, chemotaxis and motility, energy metabolism, carbonate metabolism, transport systems and cell division responded to the 17β-estradiol condition. 17β-estradiol, known as an uncommon carbon source, has potential stress for bacterial growth and metabolism compared with glucose; cells may induce specific proteins to bio-transform it and adapt this environment.
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