Abstract
Our previous studies have demonstrated the protective effects of an edible mushroom Pleurotus eryngii against the colitis in mice. Herein, an itraq‐based proteomics analysis has been utilized to establish the potential mechanism of actions of the mushroom. Three groups of mice (10 mice per group) were treated with DSS in drinking water (the colitic positive control group), DSS in drinking water plus Pleurotus eryngii (1.5% in diet, the treatment group), or regular drinking water (the negative control group), respectively, for 40 days as we described previously. The colon mucosa of mice were collected and subjected to an itraq‐based proteomic analysis. In total, 5794 proteins were identified by LC‐ESI‐MS/MS analysis in the colon mucosal of the mice. Among them, the expression levels of 626 proteins were different (392 up‐regulated proteins and 234 down‐regulated proteins) in the colitic positive control mice in comparison with the noncolitic negative control mice. There were 303 differentially expressed proteins (107 up‐regulated and 196 down‐regulated) in the treatment group compared to that of the positive control t group. The KEGG pathways that these differentially expressed proteins involved in were mainly: Steroid hormone, Arachidonic acid, Basal cell carcinoma, Linoleic acid metabolism, Phospholipase D signaling, viral myocarditis, chemical carcinogenesis, natural killer cell mediated, thyroid hormone signaling pathways. Twenty‐five proteins that were highly upregulated in the colitic positive control group (vs. the negative control group) were found significantly down‐regulated in the treatment group (vs. the colitic positive control group), whereas, twenty‐one proteins that were highly down‐regulated in the colitic positive control group (vs. the negative control group) were found significantly up‐regulated in the treatment group (vs. the colitic positive control group). Importantly, western blot analysis validated the changes in the protein expression described above. These results demonstrated that dietary mushroom treatment reversed, at least partially, the abnormal expression of proteins in the colon mucosa of colitic mice, which may be accounted for the protective effects of Pleurotus eryngii mushroom against pathogenesis of colitis in mice. Overall, our results provided preliminary but critical information on the potential mechanism of Pleurotus eryngii mushroom in inhibiting colitis.Support or Funding InformationThis research work was funded by the National Natural Science Foundation of China under Grant No. 31471927.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Published Version
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