Abstract
Front cover: Local injection of recombinant adeno associated virus (rAAV) vectors is a powerful tool to manipulate neuronal circuits like spinal sensory circuits. Depending on whether local neurons or neurons providing input to the injection site are to be manipulated different properties of rAAV vectors are required. We have identified promoter/serotype combinations that will either optimize or minimize retrograde transduction while maintaining good local transduction. The photomicrograph shows expression of eGFP in neurons of a dorsal root ganglion (DRG) which have been transduced with a rAAV vector harboring an eGFP expression cassette by intra DRG injection. The staining reveals eGFP+ neurons (green), DRG neurons that express the subtype marker gene NF200 (red) and DRG neurons that bind the Isolectin GS-IB4 (blue). Read the full article ‘Spinal nociceptive circuit analysis with recombinant adeno-associated viruses: the impact of serotypes and promoters’ by K. Haenraets, E. Foster, H. Johannssen, V. Kandra, N. Frezel, T. Steffen, V. Jaramillo, J.-C. Paterna, H. U. Zeilhofer and H. Wildner (J. Neurochem. 2017, vol. 142 (5), pp. 721–733) on doi: 10.1111/jnc.14124
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