利用ISSR及RAPD分子標誌鑑定外來入侵植物－南美獨行菜(Lepidium bonariense L.)與獨行菜(L. virginicum L.)

Abstract

Invasion by alien species represents one of the greatest biological threats to biodiversity, second only to habitat destruction. In addition to affecting ecosystems and contributing to the extinction of native species, invasive alien species also cause major socio-economic damage and may affect human and animal health. Thus developing accurate, fast and sensitive methods to detect harmful exotic plants is one of the most important projects of plant inspection and quarantine works. Lepidium bonariense L., an annual Brassicaceae plant native to South America, has become an naturalized plant in Taiwan in recent decades. As the morphological characteristics are similar, L. bonariense L. has been easily misidentified with L. virginicum L. DNA-based molecular markers have been used to detect the genetic diversity of invaded alien species. Novel methods for the identification of L. bonariense and L. virginicum at the early stage of plant development based on Inter-Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA (RAPD) markers in this study. The results of ISSR and RAPD analysis, only #820, 823, 846, 849 ISSR UBC primers and # 16, 17, 31 RAPD primers could significantly distinguish L. bonariense and L. virginicum by 2-7 and/or 2-8 different polymorphic markers. These results indicated that ISSR and RAPD methods can rapid, accurate identification of L. bonariense and may assist the effective management of this invasive species to maintain the balance of biodiversity in agricultural ecosystems.