ISSR analysis of DNA methylation variety on immature embryos of Carya cathayensis.

Abstract

To evaluation the methylation status of different immature embryo stages of Carya cathayensis, DNA methylation variety was detected on immature embryos of Carya cathayensis, and an inter-simple sequence repeat (ISSR) analysis was performed to reveal the DNA methylation in five developmental stages of immature embryos (9-13 weeks post pollination). Results revealed 128 bands obtained from 17 primers. With development of the immature embryos, the methylation percentage from 9 to 13 weeks post pollination increased gradually from 23.4% to 25.8%, 28.9%, 40.6%, and then 42.2%. Also, total DNA methylation (7.8%, 8.6%, 16.4%, 24.2%, and 25.0%) and half DNA methylation (15.6%, 17.2%, 12.5%, 16.4%, and 17.2%) existed. Thus, there was DNA methylation variety with the development of the immature embryos of Carya cathayensis(P < 0.01).