Abstract
The aims of this study were to investigate isozymic variation among some European and American cultivated oat (Avena sativa L.) varieties and to determine parental lines for an assessment of possible relationship between these markers and quantitative traits. Starch gel electrophoresis was used to determine isozyme variation among cultivars. The research was conducted at Welsh Plant Breeding Station in 1992. The variation was found for Esterase (EST), Leucine Aminopeptidase (LAP), Diaphorase (DIA), Alcohol Dehydrogenase (ADH), and Benzyol Arginine Aminopeptidase (BAP), Peroxidase (PER) among cultivars. The most extensive variation detected for EST. It indicated that North American originated lines were more variable than United Kingdom or Northern European lines. No variation was observed for other isozyme systems among oat cultivars in this study. Five bands were visualized for Glutamase Oxaloacetate Transaminase (GOT). Phospho Gluco-Isomerase (PGI) and Superoxidase-Oxidoreductase (SOD) had one band for all lines. Two active zones were observed in the parental lines for Malic Enzyme (ME). Four bands in the first zone from origin and two bands in the second were identified. Two bands were found in all parental lines for Phospho-Glucomutase (PGM). Five oat lines N327-6, N313-2, Exeter, 78-34Cn5 and Pendragon were chosen as parental cultivars for further study to examine association between isozyme and quantitative traits. These lines differed from each other for ADH, BAP, and DIA enzyme systems.
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