Isozyme banding patterns of drought tolerant sugarcane putative mutants

Abstract

Genetic characterization based on isozyme is useful for plant breeding studies. Isozyme marker may be used to understand genetic variability of irradiated plant. The objective of this research was to recognise banding pattern variabilities of 34 putative irradiated mutants that survival drought screening, based on isozyme analysis. The research was conducted in Maret – April 2015 in Biology Laboratory, PAU IPB Bogor. Vertical polyacrylamide gel electrophoresis was used to analyse three enzymes: peroxidase (PER), Esterase (EST), Aspartate Aminotransferase (AAT). Isozyme analysis was carried out on gamma rays irradiated mutants of sugarcane “Kidang Kencana” that have been selected for drought tolerant. The result showed that there was a genetic variability on 34 putative mutant of drought tolerant sugarcane, that was shown by polymorphic banding patterns on PER and EST enzymes. AAT was monomorphic, no variation was shown amongs the mutants. PER enzyme had 17 banding patterns and EST enzyme had 7 banding patterns. Dendogram based on PER and EST on 34 putative mutants could be grouped into two clusters at 81.14% genetic similirity. The isozime analysis can be used as a specific identifier of a new mutant that will be developed as new varieties.