Abstract
Bamboos are arborescent grasses belonging to the family Poaceae. To study the genetics of bamboo specially, one has to face enormous difficulties such as rare flowering and we cannot make hybridizations as easily as like other plant taxa. Unlike other tropical bamboos, studies on hill bamboos are scarce. Arundinaria falcata, an important hill bamboo, acts as soil stabilizer in hilly terrain. This study assessed the genetic diversity of 10 accessions of A. falcata collected from different localities of Garhwal Himalayas (India) and established at Hill bamboo Germplasm at Khirsu (India), using isozyme marker with four enzyme system (peroxidase, esterase, malate dehydrogenase and malic enzyme). Isozymatic analyses were performed with polyacrylamide gels (one system), bands were scored as binary data. Cluster analyses were conducted, using Jaccard´s similarity coefficient and UPGMA method. Very high degree of similarity was reported i.e. 63- 94% among different accessions. Dendrogram revealed two major clusters with three (A8- A10) and seven (A1-A7) accessions respectively. The results obtained inferred low genetic diversity in the species and urgent need of the in situ conservation of the natural genetic resources of the A. falcata species.
Highlights
Genetic conservation programmes are directed towards the longterm preservation of genetic resources either in situ or ex situ so that the potential for continuing evolution or improvement could be sustained
Ex situ conservation includes the use of botanic landscapes, field farms, seeds shops and gene financial banks and germplasm
The young leaves of selected accessions were collected from Ringal Germplasm, Khirsu, tagged properly in polybags and stored in ice bucket till they were brought to Plant Physiology Laboratory (FRI, Dehradun)
Summary
Genetic conservation programmes are directed towards the longterm preservation of genetic resources either in situ or ex situ so that the potential for continuing evolution or improvement could be sustained. In situ conservation includes the organization and/or servicing of natural supplies where species are permitted to stay in maximum environments with the lowest of management. Ex situ conservation includes the use of botanic landscapes, field farms, seeds shops and gene financial banks and germplasm. The characterization of germplasm is required to maintain identity and purity for proper conservation and management. Various breeding strategies of plants are based on the knowledge of germplasm. Germplasm characterization is an important link between the conservation and utilization of plant genetic resources for improvement
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