Isotachophoretic determination of mobility and p ka by means of computer simulation : III. Evaluation of mobility and p ka of fifteen nucleotides and seven phosphorus oxoacids and their isotachophoretic separation

Abstract

A computational method was applied for the evaluation of the absolute mobilities ( m o) and p K a values of fifteen 5'-nucleotides and seven phosphorus oxoacids (POAs) from the observed qualitative indices, R E, obtained by isotachophoresis. The nucleotides examined were the sodium salts of AMP, ADP, ATP, CMP, CDP, CTP, GMP, GDP, DTP, IMP, IDP, ITP, UMP, EDP and UTP and the POAs were the sodium salts of hypophosphorous, phosphorous, orthophosphoric, pyrophosphoric, triphosphoric, trimetaphosphoric and tetrametaphosphoric acids. Using the evaluated m o and p K a values, the separability of seven POAs and fifteen nucleotides was assessed by computer simulation. With a leading electrolyte at pH 4.7 buffered by creatinine, the isotachophoretic separation of fifteen nucleotides and pyrophosphate, triphosphate and tetrametaphosphate ions were simulated. This was confirmed by experiments and the simulated calibration characteristics of some POAs were compared with the observed values.