Separation and Quantification for Various Phosphorus Oxoacids by Isotachophoresis

Abstract

Abstract The analytical procedure for the separation and quantification of various phosphorus oxoacids was investigated by the capillary type isotachophoresis. The various phosphorus oxoacids, such as linear-, cyclic-, and lower phosphorus oxoacids, were separated at pH 5.5 with a 0.01 mol dm−3 hydrochloric acid–histidine–0.1% triton X-100 mixture as the leading electrolyte and a 0.01 mol dm−3 hexanoic acid as the terminating electrolyte. The PU value increased in the order of P3m<P4m<P3<P2<\overset1P<\overset3P<P1. The calibration curves for the various phosphorus oxoacids were linear in the range of 0.5–3.5 μg as phosphorus oxoacids. Coefficients of variation for the measured phosphorus oxoacids were less than 2% except for P3. Separation times were approximately 15 min. This procedure was applied to the ground phoshates.