Abstract
BackgroundFungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure. These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.ResultsThis study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs). The LBPs were deproteinized using sevag method, and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined polysaccharides were obtained and named LBPs-5 and LBPs-6. Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides. The molecular weight of the two water-soluble polysaccharides were estimated to be 3.235 × 103 Da and 6.196 × 103 Da by HPGPC, respectively. HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04. The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79. Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.ConclusionsThe outcome of the study indicated that LBPs had good potential as medicine and food.
Highlights
Natural plant polysaccharides are a very important class of biological macromolecules in plants, which are composed of aldoses and ketoses through glycosidic bond, and are the basic substances that effectively maintain and ensure the normal operation of biological life activities [1
The chemical structure of fungal polysaccharides plays an important role in their biological activities, including their molecular configuration, molecular weight, branched chains, branched groups and main chains [10]
When the protein was removed, polysaccharides were partially removed at the same time
Summary
Natural plant polysaccharides are a very important class of biological macromolecules in plants, which are composed of aldoses and ketoses through glycosidic bond, and are the basic substances that effectively maintain and ensure the normal operation of biological life activities [1, 2]. Fungal polysaccharides have a variety of physiological activities and are widely used in medicine, agriculture, food and other industries [3,4,5] They have various physiological functions such as immune regulation, antitumor, antiviral and antioxidant [6,7,8,9]. Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure. These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad
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