Abstract

Pseudomonas putida PPG2 grown on valine as the principal carbon and energy source produces two species of lipoamide dehydrogenase, molecular weights 56k and 49k. Pseudomonas putida grown on glucose as the carbon source produces only the 56k lipoamide dehydrogenase. The 56k proteins from P. putida grown on glucose and valine were not separated by SDS-Page and may be the same proteins. Pyruvate dehydrogenase of mutant JS94 lacking lipoamide dehydrogenase was restored by the 56k protein but not by the 49k protein. Branched chain keto acid dehydrogenase was partially restored by the 49k protein, but not the 56k protein.

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