Isolation of tRNA yeastPhe half-molecules by gel filtration and chromatography on benzoylated deae-cellulose at high temperature

Abstract

A mixture of half-molecules of tRNA Phe from yeast, produced by chemical cleavage of the anticodon loop, was isolated by gel filtration on Sephadex G-100 at 57° and fractionated by column chromatography on benzoylated DEAE-cellulose at the same temperature. Rechromatography under identical conditions yielded two major and a minor form of the 3′-half-molecule and a major and a minor form of the 5′ half-molecule. No pronounced differences were found in the amino acid acceptor activities of all complementary fragment combinations.