Isolation of the gene encoding the major endoglucanase of Erwinia chrysanthemi. Homology between cel genes of two strains of Erwinia chrysanthemi

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The structural gene encoding the major endoglucanase (EGZ) of Erwinia chrysanthemi strain 3665 has been isolated by hybridization using a DNA fragment of E. chrysanthemi strain 3937 previously cloned in the λL47-1 vector and expressing an endoglucanase activity. Furthermore, genes homologous to celZ and celY of strain 3665 were also isolated and subcloned from strain 3937 into Escherichia coli. They were shown to encode enzymes similar to EGZ and EGY of strain 3665.