Abstract
Hybridoma cell lines were produced by fusing spleen cells from 11 mice that expressed the lpr mutation to nonsecreting SP2/0 myeloma cells. Five to 20% of the hybridoma cell lines so derived were found to secrete antibodies that recognized autologous IgG2a. Out of 168 anti-IgG2a clones tested, 4 clones produced anti-IgG2a rheumatoid factor (RF) that was itself IgG2a. Two clones recognized self IgG2a while the other two clones each recognized a nonself IgG2a allotypic determinant. These findings indicate that self-recognizing antibodies are a component, albeit a minor one, of the RF repertoire of lpr lpr mice.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
