Abstract
We have identified the Na+K+2C1--cotransporter as an NH4+ transporting protein in the myeloma cell line Sp2/0-Ag14 and the hybridoma cell line 415/5 G 5 3 F 12. The NH4+ transport was dependent on the NH4+ concentration, but only slightly dependent on the extracellular pH. Bumetanide (a specific inhibitor of the Na+K+2C1--cotransporter) reduced the transport of NH4+ by 50% in both cell lines. Added NH4C1 induced apoptosis in the myeloma cell line but not in the hybridoma cell line, thus the myeloma cells are more NH3/NH4+ sensitive than the hybridoma cells. Bumetanide counteracted NH4C1-induced apoptosis in preliminary experiments with the myeloma cell line. This indicates that the cells are negatively effected by the protein-mediated transport of NH4+.
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