Isolation of phenolic acid in Acalypha indica l plants and test total phenol also antioxidant test using DPPH method

Abstract

Acalypha indica L (Anting-anting plant) is a traditional medicinal plant that can be used as a medicine for asthma, wounds, scabies, bronchitis, diuretics, and expectorants. Acalypha indica L contains secondary metabolites including alkaloid, tannin, saponin, glycoside, steroid, phenolic, terpenoid and flavonoid. The aim of this study was to isolate of phenolic acid and test total phenol also antioxidant test with the DPPH method. The sample used was ethanol crude extract from Acalypha indica obtained by maceration method with 96% ethanol. Isolation of phenolic acid from ethanol extract was carried out by the method of acid hydrolysis (HA), alkaline hydrolysis (HB) and without hydrolysis (TH) and the separation of phenolic acid isolate was undertaken using chromatography. The total phenolic yield in Acalypha indica plants was 203,065 GAE/g. The isolation results obtained in the fractions of HA, HB and TH were 0.72 g (0.036%), 1.45 g (0,073%), 0.41 g (0.020%), respectively. The results of the TLC scanner analysis on the HA, HB and TH fractions with Rf 0.63 were thought to contain caffeic acid with 21.69%, 7.14% and 2.67% respectively. The yields of the caffeic acid content using TLC Scanner on the HA, HB and TH fractions with Rf of 0.63 were 21.69%, 7.14%, 2.67%, respectively. Antioxidant activity test on the HA, HB and TH fractions showed that IC50 values were of 127.87 ppm, 132.97 ppm, 133.68 ppm respectively.